›› 2011, Vol. 23 ›› Issue (5): 384-387.doi: 10.3969/j.issn.1004-616x.2011.05.015

• 检测研究 • Previous Articles     Next Articles

Genetic toxicity of recombinant human lactoferrin

LIU Shan，FENG Xiao-lian，LI Chen-xi，GENG Gui-ying，LIU Hai-bo，ZHI Yuan，XU Hai-bin*   

  1. Institute for Nutrition and Food Safety, China Center for Disease Control and Prevention, Beijing 100021, China
  • Received:2011-03-09 Revised:2011-05-19 Online:2011-09-30 Published:2011-09-30
  • Contact: XU Hai-bin

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Abstract: OBJECTIVE: To evaluate genetic toxicity of recombinant human lactoferrin (rhLf) expressed in milk from genetically modified cow. METHODS：In Ames test，TA97，TA98，TA100，and TA102 strains were treated with 62，185，556，1 667 and 5 000 μg recombinant human lactoferrin (rhLf) per plate. In mice bone marrow cell micronucleus test and mice sperm abnormality test，1.88，3.75 and 7.50 g/kg rhLf groups and control groups were set. RESULTS：Back mutation colonies of rhLf groups did not exceed more than twice that of the control in Ames test and there was no dose-response relationship. In mice bone marrow cell micronucleus test，no significant difference was found in PCE/RBC and micronucleus rate between rhLf-treated groups and negative control group. In mice sperm abnormality test，sperm abnormality rate of rhLf-treated groups was lower than that of negative control. CONCLUSION：No genetic toxicity was observed in recombinant human lactoferrin in this study.

Key words: genetic toxicity, recombinant protein, human lactoferrin